Active Immunization with Pasteurella multocida Lysate Elicits Antibody that Protects Rabbits against Virulent Pasteurella multocida and Protects Mice by Passive Immunization
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چکیده
منابع مشابه
Passive Immunization of Pasteurella multocida Infected Rabbits
We raised neutralizing antibodies against Pasteurella multocida (PM) in the serum of rabbits and used these antibodies as immunotherapy in PM-infected rabbits. For this purpose five rabbits were vaccinated three times at 21 days interval, using formalized oil base haemorrhagic septicaemia vaccine (OBHSV). A high titer (256 IHA units) of anti-Pasteurella multocida-antibodies in blood serum was d...
متن کاملA monoclonal antibody against a Pasteurella multocida outer membrane protein protects rabbits and mice against pasteurellosis.
Monoclonal antibodies (MAbs) directed against the 37.5-kDa outer membrane protein were produced by fusing myeloma cells with spleen cells obtained from mice immunized with a pathogenic strain of Pasteurella multocida isolated from a rabbit. Desirable MAbs were selected by enzyme-linked immunosorbent assay, whole-cell radioimmunoprecipitation (WC-RIP), and Western blot (immunoblot) analysis. WC-...
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Potassium thiocyanate extracts of a virulent Pasteurella multocida 3:A rabbit isolate were prepared and used as a vaccine in rabbits. The extract contained protein, carbohydrate, hyaluronic acid, lipopolysaccharide, DNA, and RNA. The protein and lipopolysaccharide profiles of the extract were similar to those of the P. multocida cell membrane. Rabbits were vaccinated intranasally (i.n.) or intr...
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Different strategies have been developed to produce vaccines against Pasteurella multocida. The approach described herein involves overexpression on the bacterial cell surface of Fur-regulated IROMPs (iron-regulated outer-membrane proteins). Accordingly, the ability of fur mutants to promote heterologous protection was examined in a Swiss mouse animal model. Twofur mutants derived from P. multo...
متن کاملSiderophore production by Pasteurella multocida.
Pasteurella multocida grown under conditions of iron deprivation secreted into the culture medium a growth-enhancing factor which functioned as a siderophore. The siderophore was found to be neither a phenolate nor a hydroxamate by chemical tests and bioassays and was given the trivial name multocidin. Multocidin was partially purified and found to be a highly polar, nonaromatic, and dialyzable...
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ژورنال
عنوان ژورنال: Indian Journal of Animal Research
سال: 2021
ISSN: 0976-0555,0367-6722
DOI: 10.18805/ijar.b-4320